我国养羊业在发展的过程中，面临着羊粪的处理难题；另一方面，在农业生产中，为了追求高产，过度施肥往往会导致土壤酸化、水体富营养化、土壤有机质下降和土壤微生态失衡等一系列问题。解淀粉芽孢杆菌SQR9和哈茨木霉NJAU4742具有促进植物生长、提高作物产量和抑制土传病害的能力，在农业上具有广泛的应用。本研究通过在羊粪有机肥中添加解淀粉芽孢杆菌SQR9和哈茨木霉NJAU4742，研制新型羊粪生物有机肥，同时在东台和亭湖两处试验地评估新型生物有机肥料对辣椒的生物效应，以期在为生产低成本、高品质生物有机肥提供理论和实际支撑的同时，推进畜禽粪便的资源化利用和农业上生物有机肥部分替代化肥。获得了以下结果：

1、获得两种不同的羊粪生物有机肥，功能菌接种数量分别为：产品1: 解淀粉芽孢杆菌SQR9（接种后数量为2×108 CFU/g）+哈茨木霉NJAU4742接种量（接种后数量为2×107 CFU/g），产品2：解淀粉芽孢杆菌SQR9（接种后数量为2×108 CFU/g）。两个产品在货架期内有效活菌数均≥2×107 CFU/g，符合生物有机肥行业标准（NY 884-2012）。

2、试验点I（东台）和试验点II（亭湖）的田间试验结果表明，施用产品1羊粪生物有机肥能显著（P<0.05）促进辣椒的生长，提高辣椒产品，与未施肥（CK）、单施化肥（CF）和施用羊粪有机肥（AOF）相比，羊粪生物有机肥（BOF）处理亩产量分别增加403.9~506.9 kg,136.4~190.1 kg,130.4~172.1 kg，增产率达5.1%~22.9%；与CK和CF处理相比，BOF处理株高分别提高12.1%和11.6%，茎粗分别提高11.2%和8.8%。

3.施用羊粪生物有机肥可提高辣椒的品质。在试验点I:“馕播王”生物有机肥处理（NO.I）的蛋白含量显著高于CK和CF处理，AOF、BOF和NO.I处理的维生素C含量显著高于CK和CF处理，CK、BOF和NO.I的硝酸盐含量显著低于其他处理。试验点II:各处理的蛋白含量和维生素C含量无显著差异，AOF、BOF和NO.I处理在可溶性糖含量上显著高于其它处理，而CF处理硝酸盐含量显著高于其他处理。

4、施用羊粪生物有机肥能增加土壤中可培养细菌、放线菌和真菌数量，降低尖孢镰刀菌数量，增加土壤碱解氮、速效磷和速效钾含量，从而改善辣椒生长期间土壤微生物区系和提高土壤速效养分含量。

综上所述，以腐熟羊粪堆肥、酸解全价氨基酸、解淀粉芽孢杆菌SQR9和哈茨木霉NJAU4742为原料，制得羊粪生物有机肥符合生物有机肥行业标准（NY 884-2012）且施用羊粪生物有机肥能够显著提高辣椒产量，增加辣椒株高和茎粗，改善辣椒品质，优化土壤微生物，增加辣椒生长期间土壤速效养分含量。

In the process of development of sheep breeding industry in China, it is faced with the difficult problem of dealing with livestock manure. In pursuit of high yield in agriculture, excessive application of chemical fertilizers often leads to a series of environmental problems, such as soil compaction, eutrophication of water, soil organic matter decline, etc. Bacillus amyloliquefaciens SQR9 and Trichoderma harzianum 4742 have the ability to promote plant growth, increase crop yield and inhibit soil-borne diseases, so they are widely used in agriculture. This research through the solution of starch is added in the dung Bacillus amyloliquefaciens SQR9 and Trichoderma harzianum 4742, developed new sheep droppings compound biological organic fertilizer, at the same time two testbed to evaluate new biological organic fertilizer to the biological effect of chili, biological organic fertilizer with high quality and lower cost for production to improve the theoretical and practical support and promote the utilization of poultry and animal feces and agricultural portion of biological organic fertilizer instead of chemical fertilizer products. We can draw conclusions as follows:

1. Two different kinds of organic manure were obtained, and the inoculation quantity of functional bacteria were as follows: product 1: SQR9 of Bacillus amylolyticus (2×108 CFU/g) + Trichoderma Hartz NJAU4742 (2×107 CFU/g), and product 2: SQR9 of Bacillus amylolyticus (2×108 CFU/g).The effective viable bacteria number of both products in the shelf life is ≥2×107 CFU/g, which conforms to the industry standard of biological organic fertilizer (NY 884-2012).

2. Experimental field I (Dongtai) and II (Tinghu)’s results show that the application of dung biological organic fertilizer could significantly (P < 0.05), promote the growth of pepper, and improve the chili products, and no fertilizer (CK), only chemical fertilizer (CF), and organic manure of sheep (AOF) compared to sheep manure biological organic fertilizer (BOF) process yield were increased by 403.9~506.9 kg, 136.4~190.1 kg, 130.4~172.1 kg, increase rate of 5.1%~22.9%; Compared with CK and CF treatment, plant height of BOF treatment increased by 12.1% and 11.6%, and stem diameter increased by 11.2% and 8.8%, respectively.

3. The application of sheep manure bio-organic fertilizer can improve the quality of pepper. In experimental field I, the protein content of No. I bio-organic fertilizer treatment (No. I)was significantly higher than that of CK and CF treatment, the Vc content of AOF, BOF and No. I treatment was significantly higher than that of other treatments, and the nitrate content of CK, BOF and No. I treatment was significantly lower than that of other treatments. In experimental field I: there was no significant difference in protein content and Vc content in each treatment. The soluble sugar content of AOF, BOF and NO. I treatment was significantly higher than that of other treatments, while the nitrate content of CF treatment was significantly higher than that of other treatments.

4. Application of bio-organic fertilizer can improve soil microbial flora and increase the content of available nutrients in soil during the growth of pepper, increase the number of culturable bacteria, actinomycetes and fungi in soil, reduce the number of fusarium oxysporum, and increase the content of alkali-hydrolyzed nitrogen, available phosphorus and available potassium in soil.

Above all, sheep dung to finished compost, acidolysis full amino acid, SQR9 and Trichoderma harzianum NJAU4742 as raw material, sheep manure biological organic fertilizer was made by the biological organic fertilizer industry standards (NY/T 884-2012) and the application of sheep manure biol-organic fertilizer can significantly improve the hot pepper production, increase the pepper plant height and stem diameter, improving the quality of hot pepper, optimize the soil microflora, add chili soil available nutrient content during growth period.

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