风味是评价肉品品质的因素之一，而肌苷酸又是影响风味的主要物质基础之一。本研究以快大青麻鸡和灵山土鸡为研究对象，利用高效液相色谱技术测定了其胸肌肌肉的肌苷酸含量；利用分子生物学手段对肌苷酸合成代谢中的关键酶基因AMPD1的单核苷酸多态性进行了筛查，并对其中的3个SNP位点进行群体遗传结构及其与肌肉肌苷酸含量关系的分析；同时运用实时荧光定量PCR技术分析了AMPD1基因在肉鸡不同组织中的表达规律。主要研究结果如下： 1. 胸肌肌肉中肌苷酸含量的测定与分析 　　　运用高效液相色谱技术测定和分析灵山土鸡和快大青麻鸡共计116只鸡胸肌的肌苷酸含量，结果发现灵山土鸡的肌苷酸含量极显著高于快大青麻鸡（P＜0.01）；且在两品种中，母鸡的肌苷酸含量均高于公鸡。 2. 鸡AMPD1基因5’调控区和编码区SNPs筛查 利用DNA池结合直接测序的方法首次对鸡AMPD1基因的5’侧翼远端调控序列和15个外显子序列进行SNPs全面筛选，发现在灵山土鸡和快大青麻鸡中都存在10个SNPs，分别为C-555A、T-350C、C-214G、G4064A、T5288C、A5573G、T6751C、G6805A、C9228T和C9306T，其中外显子8中的T6751C为错义突变，导致原来的丝氨酸（Ser）变为半胱氨酸（Cys），该突变只轻微改变了蛋白质的理化性质，而未改变其二级和三级结构。 3. 鸡AMPD1基因的遗传多态性及其与IMP含量的相关性分析 　　　运用Bi-PASA和PCR-RLFP技术对AMPD1基因的第4064、第5573和第6805三个多态位点进行分析，发现三位点发生的均是嘌呤间的转换，且三种基因型在两个鸡种中均有分布，三个位点的多态信息含量均位于0.25和0.5之间，属于中度多态；杂合度和有效等位基因数均不高，遗传一致。 　　　分析AMPD1基因不同位点不同基因型与肌苷酸含量的关系，结果表明：对于SNP4064G-A，灵山土鸡的AA型个体IMP含量显著高于GG型个体（P＜0.05），AG型个体高于GG型个体，但差异不显著；而快大青麻鸡三种基因型间彼此均无差异；两品种鸡的公母鸡群体中各基因型间也无差异。对于SNP5573A-G，两品种鸡的三种基因型间彼此差异均不显著，两鸡种中不同性别鸡各基因型间也无差异。对于SNP 6805G-A，灵山土鸡的AA型个体IMP含量显著高于AG型个体（P＜0.05），GG型个体高于AA型个体，但差异不显著；而快大青麻鸡AG型个体的IMP含量显著高于AA型个体（P＜0.05），GG型个体高于AG型个体和AA型个体，但差异不显著；在灵山土鸡公鸡群体中，未检测到GG型个体，AA型个体的IMP含量显著高于AG型个体（P＜0.05），在母鸡群体中，AA型个体显著高于AG型个体（P＜0.05）但与GG型个体差异不显著；在快大青麻鸡公鸡群体中，GG型个体的IMP含量高于AG型个体和AA型个体，但差异不显著，AG型个体IMP含量显著高于AA型个体（P＜0.05）；在母鸡群体中，GG型个体高于AG型个体和AA型个体，但差异不显著。 4. AMPD1基因在肉鸡不同组织中的表达规律 　　　利用荧光定量PCR技术研究AMPD1基因在鸡的11种组织中的表达情况，结果表明：AMPD1基因在腹脂中表达量最高，其次是皮脂、胸肌、腿肌和肝脏，在心脏、肾脏和肌胃中表达量相对较少，在腺胃、脾脏和肺脏中几乎不表达。

Flavor is one of the elements influncing the meat quality and inosine monophosphate acid (IMP) is one of the key components for meat flavor. Our study determined IMP content of the breast muscle from the Lingshan chickens and Fast Partridge using high performance liquid chromatography technology (HPLC). At the same time, we identified the single nucleotide polymorphisms (SNPs) of AMPD1 gene which is one of the key genes in anabolic metabolism of IMP and analyzed the population genetic structure and the correlation with IMP of three SNPs. At last, we examined their expression in eleven tissues by real-time fluorescence quantitative PCR. The main results were as follows: 1. Determination and analysis of the IMP content of breast muscle 　　　The IMP content of breast muscle of the Lingshan chickens and Fast Partridge with a total number of 116 was determined using HPLC. The IMP content of Lingshan chickens was significantly higher than that of Fast Partridge(P＜0.01) and the IMP content of the females was higher than that of the males for two breeds. 2. SNPs scanning of 5'UTR and coding region of AMPD1 gene 　　　We scanned SNPs of 5'UTR and other fifteen exons of AMPD1 gene from DNA pools by sequencing. Ten SNPs were found in Lingshan chickens and Fast Partridge and they were C-555A、T-350C、C-214G、G4064A、T5288C、A5573G、T6751C、G6805A、C9228T and C9306T ; The SNP T6751C in Exon 8 was missense mutation, resulting in the change of serine (Ser) to the Cysteine (Cys). The missense mutation altered slightly the physicochemical properties of AMPD1 protein but did not change the secondary and space structure. 3. Analysis of genetic polymorphisms of AMPD1 gene and its correlation with IMP content By Bi-PASA and PCR-RLFP technology, we analyzed 4064th、5573th and 6805th of AMPD1 gene and found that they were all purine conversion. Three genotypes were found in each site in the two breed. Polymorphic information contents of the three sites belonged to moderate polymorphism (0.25 ﹀