日本脑炎（Japanese encephalitis, JE）是由日本脑炎病毒（Japanese Encephalitis Virus，JEV）引起的中枢神经系统的急性传染病。本病在世界许多国家广泛存在,不但对养猪业造成了巨大的经济损失, 而且严重威胁着人类的健康。目前对乙型脑炎的治疗仍然没有行之有效的方法,而且对待本病主要还是以免疫接种疫苗的方式进行预防。现在世界上预防本病使用的疫苗主要是一些传统的灭活疫苗和弱毒疫，这些传统疫苗存在着一些自身的弊端，如灭活苗效果不是很强，减毒苗存在毒力返强的危险等。新型有效的乙脑疫苗的研究急需进行。同时，急需研制出安全、高效、新型的抗病毒和免疫增强剂用于增强机体的抵抗力和提高疫苗的保护率。干扰素和热休克蛋白70都是生物活性蛋白，在免疫反应中具有重要的作用。论文对猪β干扰素的生物学活性进行研究，并比较研究了两种细菌Hsp70和猪β干扰素作为核酸佐剂的免疫增强效果。

Japanese encephalitis (Japanese encephalitis, JE) is an acute infectious disease of central nervous system caused by the Japanese encephalitis virus (Japanese Encephalitis Virus, JEV). The disease widespread in many countries, not only on the pig industry caused huge economic losses, but also a serious threat to human health. There is no effective treatment methods for JE, and treatment of this disease is primarily based on the way immunization vaccines for prevention. Now the world's vaccine to prevent this disease is mainly used in some of the traditional inactivated vaccine and the attenuated vaccine. There are some drawbacks of these traditional vaccines, such as the inactivated vaccine effect is not very strong, attenuated vaccine there is a strong risk of toxicity to return and so on. New and effective JE vaccine is urgently needed. Meanwhile, the urgent need to develop a safe, efficient, new anti-virus and immune enhancer for enhancing the body's resistance and improving vaccine protection rate. Interferon and heat shock protein 70 are biologically active proteins playing an important role in the immune response. In this thesis, the biological activity of swine interferon β, and a comparative study of immune-enhancing effects among the two types of bacterial Hsp70 and swine interferon β as nucleic acid adjuvant have been done.