商品中动物源成分的掺假、冒充、瞒报等关系食品安全的事件屡禁不止，损害着消费者的权益，影响着市场秩序。疯牛病的爆发，让人们开始重新思考饲料中添加动物源成分的可行性。因此，商品之中动物源成分的鉴定显得越来越重要。随着分子生物学技术的发展，微卫星DNA 已被深入了解，微卫星DNA 遵循孟德尔遗传定律，可以稳定遗传，被广泛应用于群体遗传研究，但是关于应用微卫星标记技术鉴别肉品的研究还鲜有报道。运用微卫星标记技术建立一种快速、有效的动物源成分的检测方法，确定动物成分的物种，能帮助质检人员提高工作效率降低成本，快速控制动物源性商品的随意流通，扩大食品安全控制范围，满足市场发展的检测需要。 本试验应用微卫星标记技术鉴别商品中常见的动物源性成分。采集市场上常见的猪、牛、绵羊、山羊、鸭、鹅、鸡、狗为试验对象，从Gnenbank 中初选出鸭、猪、牛、绵羊四种动物的微卫星标记，通过对微卫星标记的序列设计引物，然后进行PCR-凝胶电泳，筛选出每种动物具有种内通用、种间特异的微卫星标记用以鉴定。试验结果表明：在猪、牛、绵羊、山羊、鸭、鹅、鸡、狗的动物源性成分中鉴定鸭源性成分采用鸭的特异性微卫星标记AJ272583，扩增的目的片段为267 bp；鉴定牛源性成分采用牛的特异性微卫星标记AB463，扩增的目的片段为178 bp；鉴定绵羊源性成分采用牛的绵羊的特异性卫星标记x89268，扩增的目的片段为207 bp；鉴定猪源性成分采用猪的特异性微卫星标记EF046，扩增的目的片段为212 bp。 分别应用鸭的微卫星标记AJ272583、牛的微卫星标记AB463、绵羊卫星标记x89268、猪的微卫星标记EF046 进行荧光定量PCR，得到 鸭源性成分肉的百分含量公式为 C = 10[ ( Ct- 17.77) /-3.3844] × 100%； 牛源性成分肉的百分的含量的公式为 C = 10[ ( Ct- 16.895) /--3.1275] × 100%； 绵羊源性成分肉的百分的含量的公式为 C = 10[ ( Ct- 18.629) /-3.2525] × 100%； 猪源性成分肉的百分的含量的公式为 C = 10[ ( Ct-19.389) /-3.1439] × 100%。 实时荧光PCR 法能够对混合肉品中鸭、牛、绵羊、猪源性成分进行快速准确地定性、定量分析，可以作为市场监督和检验鉴定的可行性方法。

Lots of food adulterated, and imitationed enents take place around our life, which damaged the interests of consumers and affected the market order. Because of the outbreak of mad cow disease, people began to rethink the feasibility if animial-derived ingredients added into the feed. The identification of ingredients of animial origin became increasingly impport. With the development of molecular biology techniques, microsatellite DNA has been in-depth understanding, and widely used in population genetic studies ,because it follows Mendel's law of inheritance, can be stably inherited, but on the use of microsatellite DNA identification of meat. Establishing a rapid and effective identification of animal derived materials by microsatellite to determine the species became very important. For example: It can help impove the effective of food identification; rapid control of animal-derived free circulation of goods; expanding food safety control, to meet the needs of market development testing. This test identify the common animal-derived ingredients by use of microsatellite markers. Collecting pork, beef, sheep meat, goat meat, duck, goosen, chicken and dog meat as research objects. Picking out microsatellite markers of duck, pig, bovine and sheep from Gnenbank, designing primers based on microsatellite marker sequence, then then PCR-gel electrophoresis, screening intraspecies general, species specific microsatellite markers for identification.The results are as follows: In the identifacation of pigs, cattle, sheep, goats, ducks, goose, chicken, dogs animal derived components it can be used the duck 's specific microsatellite markers of AJ272583, which the amplified target fragments are 267 bp; the bovine's specific microsatellite markers of AB463, which the amplified target fragments are 178 bp; the sheep's specific microsatellite markers of x89268, which the amplified target fragments are 207 bp; the pig's specific microsatellite markers of EF046, which the amplified target fragments are 212 bp. Make use of duck microsatellite markers of AJ272583, bovine microsatellite markers of AB463, sheep microsatellite markers x89268, pig microsatellite markers of EF046 for fluorescence quantitative PCR. We can get the meat source component content of the formulas , the duck is: C = 10 [ ( Ct - 17.77 ) / 3.3844 ] ×100% the beef is: C = 10[ ( Ct- 16.895) /--3.1275] ×100% the sheep is: C = 10[ ( Ct- 18.629) /-3.2525] ×100% the pig is: C = 10 [ ( Ct-19.389 ) / 3.1439 ] ×100% This method can provide a rapid and accurate way for detection and analysis of the adulteration of duck, beef, mutton or pig powder,which can be used as a new evidence for market supervision and administration.