支气管败血波氏杆菌(Bordetella bronchiseptica,Bb) 是一种重要的病原菌，可广泛感染家畜、野生动物和实验动物呼吸道上皮，能引起猪、马、猫、狗、兔、小鼠、豚鼠等哺乳动物的呼吸道疾病，常导致感染动物发生鼻炎和支气管肺炎。人也有感染该菌的报道，尤其是小孩和免疫力缺陷的人群。该菌在兔群中感染率非常高, 危害家兔健康，严重影响实验兔质量和科学研究。 皮肤坏死毒素(Dermonecrotic toxin，DNT)是Bb的主要毒力因子，属于A-B型的毒素，具有典型的A-B两个亚单位。研究表明，N端是受体结合位点或中和位点，相当于B亚单位；C端是催化位点或活性中心，相当于A亚单位。因此本试验分别将结合位点和催化位点命名为DNT1、DNT3，进行了克隆、表达及其免疫保护性研究；同时本研究还利用重组蛋白DNT1和DNT3分别作为包被抗原建立了检测支气管败血波氏杆菌血清抗体的间接ELISA诊断方法。

Bordetella bronchiseptica (Bb) is an important pathogenic bacterium which can cause acute and chronic respiratory system disease. Bb can infect numerous animals, such as swine, horse, cat, canine, rabbit, mouse, cavia cobaya and so on. It always result in rhinitis and bronchial pneumonia of the infect animals. Human beings are also infected by Bb, especially children and immune deficient individuals. The high infection rate in rabbits impact rabbit product industry seriously, and affect the quality of experiment rabbits used for research. Dermonecrotic toxin (DNT) is the main virulence factor of Bb with two typical subunits A-B, belonging to A-B type toxin. Studies have shown that, N-side is a receptor-binding site or the counteractive site, which is the equivalent of B subunit; C-side is the catalytic site or active center, which is the equivalent of A subunit. Therefore, this experiment, respectively, named the binding site and catalytic site DNT1, DNT3, carried out cloning, expression and immuno-protection study; at the same time, this study also used the recombinant protein DNT1 and DNT3 respectively as coating antigen in order to establish an indirect ELISA diagnostic method for detection of bronchial defeat Blood Bordetella serum antibody.