中文题名: | 利用F2群体构建不结球白菜遗传连锁图谱 |
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学号: | 2006104044 |
保密级别: | 公开 |
论文语种: | chi |
学科代码: | 090202 |
学科名称: | 蔬菜学 |
学生类型: | 硕士 |
学位: | 农学硕士 |
学校: | 南京农业大学 |
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研究方向: | 遗传育种与分子生物学 |
第一导师姓名: | |
第一导师单位: | |
完成日期: | 2009-06-15 |
答辩日期: | 2009-06-15 |
外文题名: | CONSTRUCTION OF A GENETIC LINKAGE MAP AND MAPPING QTL OF BOLTING CHARACTER USING F2 POPULATION IN NON-HEADING CHINESE CABBAGE |
中文关键词: | |
外文关键词: | Non-heading Chinese cabbage (Brassica campestris ssp. chinensis) ; F2 population ; Genetic linkage map ; QTL mapping ; Bolting character |
中文摘要: |
本文构建了一张不结球白菜连锁遗传图谱,并在此基础上对不结球白菜抽薹性状进行了QTL分析。其主要内容如下:
首先,本文以不结球白菜N29和N14为亲本,构建了包括300个单株的F2作图群体,并应用SSR分子标记,通过Joinmap3.0软件分析,得到一张不结球白菜分子遗传图谱。图谱总长度604.7 cM,包括11个连锁群,72个多态性标记,其中偏分离标记32个,占44%。每个连锁群上的标记数在2-21之间,连锁群长度在22.1-138.7 cM的范围内,平均图距在6.6-19.4 cM之间,总平均距离8.4cM。在本研究结果的基础上可继续增加分子标记数目来不断完善不结球白菜遗传连锁图谱,更好地服务于农艺性状的定位工作。
其次,利用已构建的包括72个分子标记的不结球白菜分子遗传图谱,采用复合区间作图法(CIM)对不结球白菜抽薹性状进行了QTL定位和遗传效应分析,共得到与和抽薹日数相关的QTL4个,与开花日数相关的QTL5个,用六级分级方法检测到QTL4个,用四级分级方法检测到QTL2个。这将为不结球白菜抽薹性状分子标记辅助选择育种奠定基础。
最后,以包括186个分子标记的不结球白菜遗传连锁图谱为框架,对由112个不结球白菜株系组成的‘暑绿’双单倍体(DH)群体,采用复合区间作图法,检测了控制不结球白菜叶片脯氨酸含量和可溶性蛋白含量的数量性状基因位点(QTL)。检测到1个控制不结球白菜叶片脯氨酸含量的QTL,位于第5连锁群上,与分子标记C20相距7.2cM,LOD值为2.04,加性效应和贡献率分别为8.32%和0.37。检测到1个控制不结球白菜叶片可溶性蛋白含量的QTL,位于第3连锁群上,与分子标记AE15-3相距0.8cM,LOD值为2.43,加性效应为8.12%,贡献率为0.004。
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外文摘要: |
This objective of this paper was to construct a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp.chinensis), and mapping the QTLs for bolting character. The result were as follows:
Firstly,We constructed a F2 population consisting of 300 lines from the cultivar of non-heading Chinese cabbage N29 and N14, and achieved a genetic linkage map of non-heading Chinese cabbage, which was based on SSR molecular marker using Joinmap 3.0 software. The map covered 604.7cM, and contained 11 linkage groups and 72 polymorphic markers, in which there were 32 distorted markers (44%). The number of markers in each linkage group varied from 2 to 21, the length of linkage groups was 22.1 -138.7 cM, the mean interval distance was from 6.6 cM to 19.4 cM, and the total mean interval was 8.4 cM between markers. Using information obtained in this investigation, the development and mapping of more markers could be substantially increased in the quality of existing map, thereby providing the possibility for the future localization of various loci of important agronomic characters
Secondly, With composite interval mapping (CIM) method, a molecular genetic linkage map of non-heading Chinese cabbage were adopted to map and analyses QTL controlling the bolting character including the days of bolting, the days of florescence, six degree classing method, four degree classing method. The number, location, explained variation and additive effect of QTL were determined. Four QTL were detected for controlling the days of bolting, five QTL for the days of florescence, four QTL for six degree classing method and two QTL for four degree classing method. These results are fundamentals for molecular assisted selection of quality-related traits in non-heading Chinese cabbage breeding.
Finally, Based on a genetic linkage map of non-heading Chinese cabbage with 186 molecular markers, the composite interval method has been applied to a DH population derived from F1 hybrid variety ‘shulü’ to detected the QTL that controls the leaf proline content and soluble protein content. The result revealed one QTL for proline content in leaves, flanked by the molecular marker ‘C20’ with a distance of 7.2cM, and its additive effect is 0.37. The percent of explained using a LOD 2.04 reached to 8.32%. One QTL which controls the leaf soluble protein content has been detected on the third linkage group in the distance of 0.8cM with the molecular marker AE15-3, and that the LOD is 2.43.The additive effect is 8.12%, and the percent of explained reached 0.004.
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中图分类号: | S634.3 |
馆藏号: | 2006104044 |
开放日期: | 2020-06-30 |